Daniel Cervantes Laurean
1060 Western Av Brookings, SD 57006
dcervanteslaurean@yahoo.com     School: 605 688 6732     Home: 714 442 7601
Education
 
University of North Texas, Denton, TX
PhD - Biochemistry, August 1992
Doctoral Dissertation in the Department of Biochemistry & Molecular Biology. Doctoral Dissertation: Preparation and Characterization of Model Conjugates for the Study of Proteins Modified by ADP-Ribose.


 
Michoacana University, Morelia
Bachelor of Science - Pharmacobiology sciences, August 1985
B. S. Thesis: Transpositions in the mesilic diester of longipin-2-en-7b, 9a-diol-1-one.
Teaching Experience
 
Present Instructor of biochemistry for undergraduate and graduate students at SDSU from 2004 to present
Experience
 
South Dakota State University, Brookings, SD August 2004 - Present
Assistant Professor
Perform both independent and collaborative research in the area of protein damage and its relation to diabetes complications. Specifically, I am studying the role of oxidative stress induced by hyperglycemia in the disturbance of genomic integrity and the development of diabetes complications.
Teach graduate and undergraduate biochemistry.
Write manuscripts for publication, submit grant applications and patents, and present my research in scientific meetings.
Mentor graduate and undergraduate students
 
University of Arizona, Tucson, AZ August 1999 - July 2004
Assistant Research Scientis
Performed both independent and collaborative research in the area of skin protein photodamage. Specifically, I studied the role of the 20S proteasome in the nuclear proteolytic degradation of oxidized protein/s in skin cells in tissue culture and animal models after UVA irradiation and using biomarkers to assess overall protein oxidation. Other projects I worked on included the mechanistic study of poly ADP-ribose glycohydrolase. This enzyme in combination with poly ADP-ribose polymerase (PARP) are involved in DNA repair and apoptosis.
 
National Institutes of Health, Bethesda, MD May 1995 - July 1999
Intramural Research Training Fellowship
Worked in the pulmonary branch doing collaborative work with the Biochemistry Division to investigate the role of oxidation of alpha1-antitrypsin in lung disfunction after hyperoxia and smoking. In this study broncoalveolar lavage from healthy volunteers was obtained and alpha-1 antitrypsin was immunopurified to study the effect of methionine oxidation in its activity.
 
University of Kentucky, Lexington, KY August 1992 - April 1995
Research Associate
Postdoctoral work in the area of protein oxidation (protein glycation). This research involved the role of ADP-ribose glycation in disruption of genomic integrity and its possible role in aging and cancer.
Honors and awards
 
• Scholarship Grant by the Noble Center for Biomedical Research, Oklahoma Medical Research Foundation, Oklahoma City (1990)

• Intramural Research Training Award At the National Institutes of Health, National Heart, Blood and Lung Institute, Bethesda, Maryland (1995-1999)

• Governor’s grant for 40,000.00 (2006-2007)
Seminars and Oral Presentations
 
• University of Nebraska, Lincoln NE 01-17-2006
“The 20S proteasome in its role in the cellular response to oxidative stress”

• University of Guanajuato, Guanajuato, Gto. Mexico. 05-13-2005
“New proteasome function in oxidative stress”

• Creighton University, Omaha NE 02-24-2005
“Histone carboxymetylation and proteasome response to oxidative stress”

• North Dakota State University, Fargo, ND. 02-17-2005
“The 20S proteasome role in coping with oxidative stress in skin cells”

• South Dakota State University, Brookings, SD. 04-09-2004
“Oxidative stress induced carboxymethylation of histones and nuclear proteasome activation in human keratinocytes”

• Touro University, Mare Island, CA 05-12-2004
“Human HaCat keratinocytes after glyoxal treatment induces CML-modified histone formation and activation of the nuclear proteasome”

• Pikeville College of Osteophatic Medicine, Pikeville, KY 05-27-2004
“Carboxymethylation of histones after glyoxal treatment of keratinocytes”

• University of Kentucky (College of Pharmacy), Lexington, KY 12-12-1998
“Oxidation of human α-1-antitrypsin plays a role in its biological activity”

• National Mexican Congress of Biochemistry in Merida, Yuc. Mexico. 10-14-1998
“Oxidation of Methionine 351 and 358 inhibits the activity of human α-1-antitrypsin”

• Mexican Congress of Biochemistry in San Luis Potosi, SLP, Mexico. 07-14-1991
“Preparation of low molecular weight models to study mono ADP-ribosylation of proteins”

• Noble Center for Biomedical Research, Oklahoma City, OK. 04-07-1990
“Propionyl ADP-ribose as a low molecular weight model to study poly ADP-ribose metabolism”
Patents and Publications
 
Publications

1. Roman, L. U., del Rio, R. E., Hernandez, J. D., Cerda, C. M., Cervantes Laurean, D., Castaneda, R., & Joseph-Nathan, P. (1985) Structural and Stereochemical Studies of Naturally Occurring Longipinene Derivatives. J. Org. Chem. 50, 3965-3972.

2. Jacobson, M. K., Aboul-Ela, N., Cervantes Laurean, D., Loflin, P. T., Jacobson, E. L. (1990) ADP-Ribose Levels in Animal Cells in “ADP-ribosylating Toxins and G-Proteins: Insights into Signal Transduction.” Moss, J. & Vaughan, M. Eds., American Society for Microbiology, Washington, D. C., 479-492.

3. Cervantes-Laurean, D., Minter, D. E., Jacobson, E. L., & Jacobson, M. K. (1993) Protein Glycation by ADP-ribose: Studies of Model Conjugates. Biochemistry 32, 1528-34.

4. Jacobson, E. L., Cervantes Laurean, D., & Jacobson, M. K. (1994) Glycation of Proteins by ADP-ribose. Mol. Cell. Biochem. 138, 207-12.

5. Cervantes-Laurean, D., Loflin, P. T., Minter, D. E., Jacobson, E. L., & Jacobson, M. K. (1995) Protein Modification by ADP-ribose via Acid Labile Linkages. J. Biol. Chem. 270, 7929-36.

6. Jacobson, M. K., Cervantes-Laurean, D., Strohm, M. S., Coyle, D. L., Bummer, P. M., & Jacoson, E. L. (1995) NAD glycohydrolases in the Metabolism of Cyclic ADP-ribose. Biochemie 77, 341-44.

7. Cervantes-Laurean, D., Jacobson, E. L., & Jacobson, M. K. (1996) Glycation and Glycoxidation of Histones by ADP-ribose. J. Biol. Chem. 271, 10461-69.

8. Jacobson, E. L., Cervantes Laurean, D., & Jacobson, M. K. (1996) ADP-ribose Glycoxidation Reactions. Biological Significance of Mono-ADP-ribosylation in Animal Tissues, Hamburg-Blankenese, Germany.

9. Cervantes-Laurean, D., Jacobson, M.K., and Jacobson, E.L. (1997) “Preparation of Low Molecular Weight Model Conjugates for ADP-ribose Linkages to Protein.” Methods in Enzymology 280, 275-87.

10. Jacobson, E.L., Cervantes-Laurean, D., & Jacobson, M. K. (1997) ADP-Ribose in Glycation and Glycoxidation Reactions. In ADP-Ribosylation in Animal Tissues. H. Koch-Nolte, editor. Plenum Press, New York. 371-379.

11. Jacobson, E.L., D. Cervantes-Laurean, and M.K. Jacobson. 1997. ADP-ribose in glycation and glycoxidation reactions. Adv Exp Med Biol. 419:371-379.

12. Cervantes Laurean, D., McElvaney, N.G., & Moss, J. (1999) “Niacin” in Modern Nutrition in Health and Disease (Shils, M.E., Olson, J. A., Shike, M., & Ross A. C. Eds.) 23, 401-11

13. Cervantes-Laurean*, D., Taggart,* C., McElvaney, N.G., Kim, G., Wehr, N.B., Moss, J., and Levine, R.L. (2000) “Oxidation of Either Methionine 351 or Methionine 358 in α1-Antitrypsin Causes Loss of Anti-Neutrophil Elastase Activity.” J. Biol. Chem. 275, 27258-65.

14. Wondrak, G.T., Cervantes-Laurean, D., Jacobson, E.L., and Jacobson, M.K. (2000) “Histone Carbonylation in vivo and in vitro”. Bochem. J. 351(3), 769-777.

15. Wondrak, G.T., Cervantes-Laurean, D., Roberts, M.J., Qasem, J., Moonsun, K., Jacobson, E.L., and Jacobson, M.K (2002). “Identification of Dicarbonyl Scavengers for Cellular Protection against Carbonyl Stress”. Biochem. Pharmacology. 63, 361-373

16. Roberts, M. J., Wondrak, G. T., Cervantes Laurean, D., Jacobson, M. K., and Jacobson, E. L. (2003) “DNA Damage by Carbonyl Stress in Human Skin Cells” Mutation Research 522, 45-56.

17. Wondrak, G.T., Roberts, M.J., Cervantes Laurean, D., Jacobson, M.K., and Jacobson, E.L (2003). “ Proteins of the Extracellular Matrix are Sensitizers of Photooxidative Stress in Human Skin Cells" J Invest Dermatol 121, 578-586..

18. Bourgeois, C., Cervantes Laurean, D., & Moss, J. (2005) “Niacin” in Modern Nutrition in Health and Disease (Shils, M.E., Olson, J. A., Shike, M., & Ross A. C. Eds.) 25, 434-441

19. Daniel Cervantes Laurean, Michael J. Roberts, Elaine L. Jacobson. (2005) “Nuclear proteasome activation and degradation of carboxymethylated histones in human keratinocytes following glyoxal treatment” Free Rad. Biol. Med. 38, 786-795.

20. Daniel Cervantes-Laurean, Derek D. Schramm, Brian J. Moran, Elaine L. Jacobson, Geza G. Bruckner, and Gilbert A. Boissonneault (2006). “Inhibition of Advanced Glycation End Product Formation by Rutin Metabolites”. J. Nutr. Biochemistry 17, 531-40.


21. Daniel Cervantes Laurean, Hong Gao, Elaine L. Jacobson and Myron K Jacobson “A re-examination of the relationship of enzymes of ADP-ribose polymer metabolism”. In preparation

PATENTS

1. NIAD 215.1 (Issued) Patent Serial Number: 09/836,552 Inventors: E. Jacobson, M. Jacobson, G. Wondrak, and D. Cervantes-Laurean Title: “Method and Use of Penicillamines and Other α-Amino-β-Mercapto-Ethane Derivatives as Dicarbonyl Scavengers for Treatment of Conditions Resulting from Protein, Lipid, and DNA Damage” Filing Date: April 14, 2000 and April 16, 2001, U. S. Patent Office Date of Issuance: July 9, 2002 as U. S. Patent Number 6,417,235, University of Kentucky.

2. NIAD 215 Patent Serial Number: 60/197,216 Inventors: E. Jacobson, M. Jacobson, G. Wondrak, and D. Cervantes-Laurean Title: “Method and use of Penicillamines as Dicarbonyl Scavengers for Treatment of Conditions Resulting from Protein, Lipid, and DNA Damage” Filing Date: April 14, 2000 and April 14, 2001. University of Kentucky.

3. NIAD 215.2 (Filed) Patent Serial Number: 09/836,552 Inventors: E. Jacobson, M. Jacobson, G. Wondrak, and D. Cervantes-Laurean Title: “Method and use of α-Amino-β-Mercapto-Ethane Derivatives as Dicarbonyl Scavengers for Treatment of Conditions Resulting from Protein, Lipid, and DNA Damage” Filing Date: April 14, 2000 and March 7, 2002, University of Kentucky.