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To obtain a full-time research associate position that will maximize my leadership, organizational, and technical skills.
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December 2001 |
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Gainesville, FL |
| | Harbor Branch Oceanographic Institution |
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August 2001 - Present |
Aquaculture Laboratory Assistant
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Ft. Pierce, FL |
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| · | PCR analysis of commercial shrimp samples
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| · | Detection of viral (both RNA and DNA) disease in shrimp for shrimp farms around the world
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| · | DNA extraction via mini-preps
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| | College of Medicine Department of Molecular Genetics & Cell Science |
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September 1999 - August 2001 |
Graduate Research Assistant
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Gainesville, FL |
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| · | Analyzed the ability of V. vulnificus phage to treat and prevent disease caused by V. vulnificus in mouse model. Infected and treated mice with subcutaneous, intravenous, and intraperitoneal injections of bacteria and phage. Performed necropsy on mice.
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| · | Conducted histopathological analysis of skin lesions to determine if phage therapy had a microscopic effect on the vasculature of infected mice.
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| · | Created an invertebrate animal model of infection and treatment which included: generating spontaneous antibacterial V.v. mutant, designed a water purification/ circulation center, dissection and tissue homogenization of infected /treated oysters
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| · | Isolated, amplified, and purified V. vulnificus bacteriophage
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General Lab Responsibilities:
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| · | DNA extraction and digestion, phenol extraction, ethanol precipitation
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| · | Supervised an undergraduate staff, coordinated rotational Ph.D. project
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| · | Supervised supply purchasing, biohazard disposal, and maintained media stocks in BSL-2 lab
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| · | Organized mouse data logs using Quatro Pro. Familiar with Microsoft Office and Adobe Photoshop
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| | College of Medicine, Department of Oral Biology |
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October 1998 - May 1999 |
Lab Tech
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Gainesville, FL |
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| · | SDS-PAGE and Western blot analysis
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| · | Maintained the anaerobic growth chamber
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Organizations and Meetings
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American Society of Microbiology, Member 1999 to present
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| · | Presented at the 100th general meeting in Los Angeles, California May 2000
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| · | Attended the 101st general meeting in Orlando, Florida, May 2001
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American Association for the Advancement of Science, Member 1999 to present
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Society of Biomaterial Engineering, Member 1999
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| · | Attended the 4th annual world biomaterial conference May 2000, in Kona, Hawaii
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Poster Presentation at the annual SEA-GRANT meeting in Tampa, Florida 2000
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Lady Gator Water Polo 1995 to present
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University of Florida Intramural Sports 1995 to 1999.
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Phage Therapy Can Prevent Local and Systemic Disease Caused by Vibrio vulnificus in Iron-Dextran-Treated Mice. Cerveny, K., Duckworth, D., DePaola, A., and Gulig, P. (submitted to Infection and Immunity - under review)
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Use of Bacteriophage as Therapy for Disease Caused by Vibrio vulnificus in Iron-Dextran-Treated Mice. Cerveny KE, Doyle TJ, Escudero GM, et al. [Abstract D230] In: Abstracts Annual Meeting of the American Society of Microbiology 2000; 279
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Expression and Immunogenicity of Hemagglutinin A from Porphyromonas gingivalis in an Avirulent Salmonella enterica Serovar Typhimurium Vaccine Strain. Kozarov, E., Miyashita, N., Burks, J., Cerveny, K., Brown, T., McArthur, W., and Progulske-Fox, A. Infection and Immunity, Feb. 2000, p. 732-739
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